Agrobacterium-mediated Genetic Transformation in Cucumber (Cucumis sativus L.)
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چکیده
منابع مشابه
Agrobacterium-Mediated Transformation in Cucumber (Cucumis sativus L.)
Introduction. Cucumber (Cucumis sativus L.) is an important horticultural crop. Its fruits are used for slicing and pickling and juice extraction worldwide. In India, both the fruits and seeds have long been used in the manufacture of traditional medicines that act as skin conditioners, diuretics and body coolants. Thus, cucumber is a potential candidate for an edible vaccine (6). In India, stu...
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In this research, twenty genotypes of cucumber (Cucumis sativus) were studied that were collected from different geographical regions. The experiment was conducted as a randomized complete Block Design (RCBD) with four replications in Greenhouse. Data was collected on morphological features, yield and components of yield of cucumber which include: Total fruit yield per pickling, Fruit number pe...
متن کاملEthyl Methanesulfonate (EMS)-Mediated Mutagenesis of Cucumber (Cucumis sativus L.)
Ethyl methanesulfonate (EMS) is a stable and effective chemical mutagen. In this study, cucumber (Cucumis sativus L. cv. “Shannong No. 5”) seeds were treated by 1% EMS for 12 h, 24 h and 48 h to optimize EMS mutagenesis and determined median lethal dose of EMS (1% EMS and 24 h) for “Shannong No. 5”. After treated by 1% EMS for 24 h, 541 M1 plants were grown in greenhouse for phenotype investiga...
متن کاملGenetic Diversity and Population Structure of Cucumber (Cucumis sativus L.)
Knowing the extent and structure of genetic variation in germplasm collections is essential for the conservation and utilization of biodiversity in cultivated plants. Cucumber is the fourth most important vegetable crop worldwide and is a model system for other Cucurbitaceae, a family that also includes melon, watermelon, pumpkin and squash. Previous isozyme studies revealed a low genetic diver...
متن کاملIn vitro organogenesis and genetic transformation in popular Cucumis sativus L. through Agrobacterium tumefaciens.
The effect of growth regulators and culture conditions on the morphogenetic response of cotyledonary leaf discs was studied in popular cucumber variety (Cucumis sativus cv. Sheetal). Organogenesis was induced directly without any intervening callus phase on Murashige and Skoog medium supplemented with different concentrations of benzyladenine and indole propionic acid. Best results (93%) were o...
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ژورنال
عنوان ژورنال: American Journal of Biochemistry and Biotechnology
سال: 2007
ISSN: 1553-3468
DOI: 10.3844/ajbbsp.2007.24.32